Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.
Identifieur interne : 003093 ( Main/Exploration ); précédent : 003092; suivant : 003094Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.
Auteurs : Nicole J. Dafoe [Canada] ; Brent E. Gowen ; C Peter ConstabelSource :
- BMC plant biology [ 1471-2229 ] ; 2010.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Chimère (MeSH), Données de séquences moléculaires (MeSH), Feuilles de plante (génétique), Phloème (composition chimique), Phloème (métabolisme), Populus (composition chimique), Populus (métabolisme), Protéines végétales (analyse), Protéines végétales (composition chimique), Protéines végétales (métabolisme), Protéomique (méthodes), Séquence d'acides aminés (MeSH).
- MESH :
- analyse : Protéines végétales.
- composition chimique : Phloème, Populus, Protéines végétales.
- génétique : Feuilles de plante.
- métabolisme : Phloème, Populus, Protéines végétales.
- méthodes : Protéomique.
- Animaux, Chimère, Données de séquences moléculaires, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Animals (MeSH), Chimera (MeSH), Molecular Sequence Data (MeSH), Phloem (chemistry), Phloem (metabolism), Plant Leaves (genetics), Plant Proteins (analysis), Plant Proteins (chemistry), Plant Proteins (metabolism), Populus (chemistry), Populus (metabolism), Proteomics (methods).
- MESH :
- chemical , analysis : Plant Proteins.
- chemistry : Phloem, Plant Proteins, Populus.
- genetics : Plant Leaves.
- metabolism : Phloem, Plant Proteins, Populus.
- methods : Proteomics.
- Amino Acid Sequence, Animals, Chimera, Molecular Sequence Data.
Abstract
BACKGROUND
Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa x P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.
RESULTS
Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.
CONCLUSIONS
TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.
DOI: 10.1186/1471-2229-10-191
PubMed: 20796310
PubMed Central: PMC2956541
Affiliations:
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Gowen</name></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20796310</idno><idno type="pmid">20796310</idno><idno type="doi">10.1186/1471-2229-10-191</idno><idno type="pmc">PMC2956541</idno><idno type="wicri:Area/Main/Corpus">003084</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003084</idno><idno type="wicri:Area/Main/Curation">003084</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003084</idno><idno type="wicri:Area/Main/Exploration">003084</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.</title><author><name sortKey="Dafoe, Nicole J" sort="Dafoe, Nicole J" uniqKey="Dafoe N" first="Nicole J" last="Dafoe">Nicole J. Dafoe</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biology, University of Victoria, Victoria, BC, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Department of Biology, University of Victoria, Victoria, BC</wicri:regionArea><wicri:noRegion>BC</wicri:noRegion></affiliation></author><author><name sortKey="Gowen, Brent E" sort="Gowen, Brent E" uniqKey="Gowen B" first="Brent E" last="Gowen">Brent E. Gowen</name></author><author><name sortKey="Constabel, C Peter" sort="Constabel, C Peter" uniqKey="Constabel C" first="C Peter" last="Constabel">C Peter Constabel</name></author></analytic><series><title level="j">BMC plant biology</title><idno type="eISSN">1471-2229</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Animals (MeSH)</term><term>Chimera (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Phloem (chemistry)</term><term>Phloem (metabolism)</term><term>Plant Leaves (genetics)</term><term>Plant Proteins (analysis)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (metabolism)</term><term>Populus (chemistry)</term><term>Populus (metabolism)</term><term>Proteomics (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Chimère (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Phloème (composition chimique)</term><term>Phloème (métabolisme)</term><term>Populus (composition chimique)</term><term>Populus (métabolisme)</term><term>Protéines végétales (analyse)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (métabolisme)</term><term>Protéomique (méthodes)</term><term>Séquence d'acides aminés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Phloem</term><term>Plant Proteins</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Phloème</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Leaves</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Feuilles de plante</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phloem</term><term>Plant Proteins</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Proteomics</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Phloème</term><term>Populus</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Protéomique</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Chimera</term><term>Molecular Sequence Data</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Chimère</term><term>Données de séquences moléculaires</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa x P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</p></div><div type="abstract" xml:lang="en"><p><b>RESULTS</b></p><p>Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>TLP localization in poplar cells and tissues is complex. TLP1 is expressed predominantly in tissues with a prominent vascular system such as midveins, petioles and stems, whereas the second TLP is primarily expressed in starch-storing plastids found in young leaves and the shoot apex.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20796310</PMID><DateCompleted><Year>2010</Year><Month>10</Month><Day>04</Day></DateCompleted><DateRevised><Year>2018</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1471-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><PubDate><Year>2010</Year><Month>Aug</Month><Day>26</Day></PubDate></JournalIssue><Title>BMC plant biology</Title><ISOAbbreviation>BMC Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Thaumatin-like proteins are differentially expressed and localized in phloem tissues of hybrid poplar.</ArticleTitle><Pagination><MedlinePgn>191</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1186/1471-2229-10-191</ELocationID><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Two thaumatin-like proteins (TLPs) were previously identified in phloem exudate of hybrid poplar (Populus trichocarpa x P. deltoides) using proteomics methods, and their sieve element localization confirmed by immunofluorescence. In the current study, we analyzed different tissues to further understand TLP expression and localization in poplar, and used immunogold labelling to determine intracellular localization.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Immunofluorescence using a TLP antiserum confirmed the presence of TLP in punctate, organelle-like structures within sieve elements. On western blots, the antiserum labeled two constitutively expressed proteins with distinct expression patterns. Immunogold labelling suggested that TLPs are associated with starch granules and starch-containing plastids in sieve elements and phloem parenchyma cells. In addition, the antiserum recognized TLPs in the inner cell wall and sieve plate region of sieve elements.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">TLP localization in poplar cells and tissues is complex. 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Gowen</name></noCountry><country name="Canada"><noRegion><name sortKey="Dafoe, Nicole J" sort="Dafoe, Nicole J" uniqKey="Dafoe N" first="Nicole J" last="Dafoe">Nicole J. Dafoe</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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